All projects
Dec 2023Genetics

CRISPR Gene Protocol Development

Developed a CRISPR protocol to alter the speed of the ALDH gene pathway.

GeneticsBiologyResearch
Illustration of a PCR thermal cycler with a tube strip beside a gel electrophoresis tank showing separated DNA bands

Overview

A molecular-biology project building a CRISPR protocol aimed at the ALDH gene pathway. The work centered on designing the right primers and verifying which genes — and which edits — were present.

The protocol moved from primer design through confirmation, with RNA proteins used as part of the workflow.

What I did

  • Developed a CRISPR protocol to alter the speed of the ALDH gene pathway.
  • Created DNA allele-specific primers to identify genes present and genes that had been altered.
  • Worked with RNA proteins as part of the protocol.

Outcome

The protocol established a repeatable way to target and verify edits in the ALDH pathway, from primer design through confirmation.